SSM for education

Thursday, December 29, 2016

اس اس ام : سنه تالته ترم اول

https://www.dropbox.com/sh/866ff1fo5a6eo7n/AACHxf_TZ3Qb7TBvaOPXiu4Za?dl=0

اضغط على الصورة 😁👆👆👆👆

مجموعه تبييضات SSM قديمه لسنه 3 ترم اول

shady safwat Web Developer

Shady Safwat Mehanny, (SSM)- contact me (+2) 0127 0141 385. and at shadysafwatnobel@gmail.com

Colloid & Surface | Dr Khaled

reference of the book:
http://albumen.conservation-us.org/library/c20/shaw1992.html

shady safwat Web Developer

Shady Safwat Mehanny, (SSM)- contact me (+2) 0127 0141 385. and at shadysafwatnobel@gmail.com

Wednesday, December 07, 2016

عملى كيمياء الانسجة | ا.د/ محمد عرفة

1- H&E

Aim : demonstration of nucleus the mammalis

Section : T.S in liver of mammalia

T.S in kidney of mammalia

T.S in ileum of the mammalia

T.S in adrenal gland of the mammaila

Color : cytoplasm (red) ….. nucleus ( deep blue )

2-Hg- bromophenol blue

Aim : identification of total protein

Section : T.S in liver of Rabbit

T.S in ileum of the Rabbit

T.S in adrenal gland of the Rabbit

T.S in kidney of Rabbit

Color : cytoplasm (deep blue )

The nucleus isn't stained

The degree of color depend on the protein

Concentration in cytoplasme and cell membrane

3-Alcian blue (Cu- phathelo cyanine )

Aim : identification of acid mucopolysaccarides (mucoids)

Section : T.S in ileum of the Rabbit

Color : cytoplasm ( pale green color )……. Nucleus : not stained

4-Silver nitrate

Aim : Reveal golgi complex

Golgi complex found in cytoplasm and its depend on the physiological condition for the animal

Color : dark brown to black deposits

Section : T.S in liver of Rabbit

T.S in kidney of Rabbit

T.S in adrenal gland of the Rabbit

T.S in the Vas deferens of the Rabbit

5-PAS (periodic acid Schiff reaction

Aim : Identification of carbohydrates

Section : T.S in the liver of mammals

Colour: Cytoplasm à purple colour

Nucleus à colorless

Mechanism :-

1) Oxidation : HIO₄ is a weak oxidizing agent used to oxidize the carbohydrate to aldehyde

2) Schiff reagent preparation

6-Stain : Fulgen reactionAim : identification of DNA

Section : T.S in the testes of the mammals

Color : cytoplasm à colorless nucleus à purple color

7- Immunohistochemical method

1- in this method we aim to detect a single protein
2-this is done usinga spcific (antibody) “polyclonal - monoclonal”
3- this is (antigen-antibody) complex can be detected by two different methods

A) The direct method : at which tje flarochrome ia attachedto
the 1ry antibody can be only detected using flurocent microscope
(UV) light source

B) The indirect method : at which tje flarochrome ia attachedto the 2ry antibody
- can be only detected using flurocent microscope (UV) light source
- at which a streptavidin interactes with HRP [Horse Raddish Peroxidose]
in the presence of DAB [ Di Amino Benzidin]
and a block celover is formed at the sited expression

- TNFR2 ... Tumer Necrosis Factor Receptor 2 protein (Localized in the ONL, Outer Nuclear Layer and INL , Inner Nuclear layer) of the retina of carp larva (bony fish).

- HSP70, Heat shock protein 70 , which is localized at the OPL (outer plexiform layer) of the retina of carp larva (bony fish).