1- H&E
Aim : demonstration of nucleus the mammalis
Section : T.S in liver of mammalia
T.S in kidney of mammalia
T.S in ileum of the mammalia
T.S in adrenal gland of the mammaila
Color : cytoplasm (red) ….. nucleus ( deep blue )
2-Hg- bromophenol blue
Aim : identification of total protein
Section : T.S in liver of Rabbit
T.S in ileum of the Rabbit
T.S in adrenal gland of the Rabbit
T.S in kidney of Rabbit
Color :
cytoplasm (deep blue )
The nucleus isn't stained
The degree of color depend on the protein
Concentration in
cytoplasme and cell membrane
Aim : identification of acid mucopolysaccarides (mucoids)
Section : T.S in ileum of the Rabbit
Color :
cytoplasm ( pale green color )……. Nucleus : not stained
4-Silver nitrate
Aim : Reveal
golgi complex
Golgi complex found in cytoplasm and its depend on the
physiological condition for the animal
Color : dark brown to black deposits
Section : T.S in liver of Rabbit
T.S in kidney of Rabbit
T.S in adrenal gland of the Rabbit
T.S in the Vas deferens
of the Rabbit
5-PAS (periodic acid Schiff reaction
Aim : Identification of carbohydrates
Section : T.S in the liver of mammals
Colour: Cytoplasm à purple colour
Nucleus
à colorless
Mechanism :-
1)
Oxidation : HIO4 is a weak oxidizing agent used to
oxidize the carbohydrate to aldehyde
2)
Schiff reagent preparation
6-Stain : Fulgen reactionAim : identification of DNA
Section : T.S in the testes of the mammals
Color : cytoplasm à colorless nucleus à purple color
7- Immunohistochemical method
1- in this method we aim to detect a single protein
2-this is done usinga spcific (antibody) “polyclonal - monoclonal”
3- this is (antigen-antibody) complex can be detected by two different methods
A) The direct method : at which tje flarochrome ia attachedto
the 1ry antibody can be only detected using flurocent microscope
(UV) light source
B) The indirect method : at which tje flarochrome ia attachedto the 2ry antibody
- can be only detected using flurocent microscope (UV) light source
- at which a streptavidin interactes with HRP [Horse Raddish Peroxidose]
in the presence of DAB [ Di Amino Benzidin]
and a block celover is formed at the sited expression
7- Immunohistochemical method
1- in this method we aim to detect a single protein
2-this is done usinga spcific (antibody) “polyclonal - monoclonal”
3- this is (antigen-antibody) complex can be detected by two different methods
A) The direct method : at which tje flarochrome ia attachedto
the 1ry antibody can be only detected using flurocent microscope
(UV) light source
B) The indirect method : at which tje flarochrome ia attachedto the 2ry antibody
- can be only detected using flurocent microscope (UV) light source
- at which a streptavidin interactes with HRP [Horse Raddish Peroxidose]
in the presence of DAB [ Di Amino Benzidin]
and a block celover is formed at the sited expression
- TNFR2 ... Tumer Necrosis Factor Receptor 2 protein (Localized in the ONL, Outer Nuclear Layer and INL , Inner Nuclear layer) of the retina of carp larva (bony fish).
- HSP70, Heat shock protein 70 , which is localized at the OPL (outer plexiform layer) of the retina of carp larva (bony fish).
بنشكر دكتور صلاح على تعبة معانا جدا 😊😊😊
دة كلام فوق النمرة واحد
ReplyDeleteشالله رب يخليك
Deleteالله يا شوشو
ReplyDeleteبردو مش هبعتهملك يا جاد
Deleteالله ينور يا رجالة ..
ReplyDeleteعندي شوية ملاحظات يا ريت تأخذ في الاعتبار
1- Cu- phathelo cyanine ---- Cu- Phthalocyanine cyanine
the cytoplasm is stained with pale green / blue colour while the mucopolysaccharides stained with dark blue colour.
2- Silver Nitrate .
stains Golgi apparatus , the localization of the dark colour deposits depends on the type of the cell .
*Liver.... polygonal hepatocytes (surrounding the nucleus).
*cuboidal and columnar cells , above the nucleus.
طبعاً باقي الكومنتات في الورق
و مننساش الكومنت للكمياء النسيجية المناعية اللي كتبته على السبورة
TNFR2 ... Tumer Necrosis Factor Receptor 2 protein (Localized in the ONL, Outer Nuclear Layer and INL , Inner Nuclear layer) of the retina of carp larva (bony fish).
HSP70, Heat shock protein 70 , which is localized at the OPL (outer plexiform layer) of the retina of carp larva (bony fish).
تمام يا دكتور صلاح
Deleteجاري التعديل على الاخطاء الان .. تعبتك معايا ربنا يخليك 😊😊
عليا الطلاق علوضعك اشوشو
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